AG Junglen - Ecology and evolution of arboviruses

  • Virus diversity
  • Evolution of arthropod-associated viruses
  • Detection, isolation and characterisation of novel viruses
  • Arthropod and virus ecology
  • Geographic spread of arthropod-borne viruses

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Focus research

Our research focuses on the ecology and evolution of arthropod-associated viruses. We are particularly interested in the genetic diversity of these viruses and in ecological mechanisms that influence the geographic spread and emergence of arboviruses. We conduct fieldwork in pristine ecosystems and adjacent landscapes in Africa and Central America to investigate initial emergence processes from enzootic to epidemic transmission cycles.

Group members

Georg Eibner

I study the effect of community composition on viral infection rates in mosquitoes using mesoniviruses as an example. To perform these analyses, I sampled mosquitoes along anthropogenic disturbance gradients in rainforest and savannah regions in Uganda.

Selina Graff

I am interested in the genetic and phenotypic diversity of arboviruses in mosquito species that have been neglected so far. The studies are based on mosquitoes that I sampled in primary rainforest and savannah regions in Uganda.

Heiko Guggemos

I try to understand how the development of the world’s largest transboundary conservation area, the KAZA park, affects vector-borne disease dynamics. I conduct my research in the Caprivi strip in Northern Namibia.

Kyra Hermanns

I analyze the diversity and prevalence of RNA viruses in mosquitoes with a special focus on novel alphaviruses. Furthermore, I am interested in direct and indirect interactions between mosquito-associated viruses.

Anne Kopp

I am studying the diversity of mosquito-associated viruses originating from the New World. Currently, I am interested in insect-specific flaviviruses and the integration of flavivirus-derived endogenous elements in mosquito genomes.

Dr. Marco Marklewitz

My research focuses on the detection and characterization of previously unknown bunyaviruses and on the identification of the most important arboviroses in Kenya. I am involved in the development of diagnostic methods and in capacity building in Kenya.

Sandy Ayash

student assistant

Morris Baumgardt

student assistent

Matthias Fendt

I test biting migdes, which I collected in the KAZA-Park in Namibia, for infections with arboviruses.

  • Dr. Marco Marklewitz, Postdoc 
  • Ahmad Ghiffari, PhD Student  
  • Kyra Hermanns, PhD Student  
  • Anne Kopp, PhD Student  
  • Ramesh Pun, PhD Student 
  • Georg Eibner, PhD Student 
  • Selina Graff, PhD Student 
  • Verena Heyde, Technical assistance
  • Christian Hieke, Technical assistance

Alumni: Saba Motthaginia, Pascal Trippner, Lisa Baum, Marilene Sonja Frey, Katrin Rindt, Alexandra Hübner, Thierry Kruber, Ramona Gilles, Mario Schmitz, Dinah Henritzi, Friederike Schröder, Wolfgang Grasse, Susann Handrick, Daniel Hobelsberger, Nadine Hoffmann, Nancy Ernst, Florian Zirkel

Projects

Induction and Suppression of antiviral RNAi pathways by mosquito-specific and mosquito-borne viruses

Arthropod-borne viruses (arboviruses) are naturally maintained in an enzootic (sylvatic) cycle in which virus is transmitted during blood-feeding between arthropod vectors and amplifying vertebrate hosts. Human infections can be caused by spill-over infections or by viral adaptation to anthropophilic mosquito species and to humans as host. Evolution of such an urban transmission cycle is associated with greatly increased epidemic potential of arboviruses. With the increasing global spread of arboviruses, it is important to understand the factors that affect virus transmission by mosquitoes and to predict the potential to arboviruses to emerge into epidemic cycles. 

RNA interference (RNAi) is an important antiviral mechanism in insects, including the major vector mosquitoes, and many insect viruses evolved counter-defense mechanisms to suppress RNAi. The aim of the first funding period was to understand the role of RNAi in Flavivirus virus infection of mosquitoes. We established robust assays to identify RNAi suppressors in mosquito viruses. Using these assays, we were unable to identify RNAi suppressors in a broad panel of flavivirus family members, including mosquito-specific viruses and mosquito-borne arboviruses. We analyzed viral small RNA profiles using deep-sequencing technology and found that Culex and Aedes-tropic viruses in Aedes cells produce similar levels of small RNAs. Surprisingly in two of the analyzed members of flaviviruses we found, in addition to the production of virus-derived small interfering RNAs (siRNA) evidence for the production of small RNAs with the characteristics of piwi-associated RNAs (piRNA). 

The overall aim of this project is to understand the role of antiviral RNAi responses and viral counter-defense in establishing vector competence in mosquitoes. We will compare the induction and suppression of RNAi among representatives of the Bunyaviridae family that differ in their mode of transmission, vector tropism, and sylvatic or epidemic origin, as a parallel example to the Flaviviridae that were analyzed in the first funding period. Moreover, we will analyze small RNAs from naturally infected mosquitoes and viral RNAi suppression of novel viruses that are identified in an ecological survey of wild-caught mosquitoes within the framework of SPP1596.

Arboviruses are an important group of human and animal pathogens and their emergence and re-emergence requires thorough understanding of vector transmission. This proposal will have a major impact on our understanding of vector competence. Furthermore, our results will contribute to our ability to predict the ability of arboviruses to cross the species barrier and the potential of known and novel sylvatic arboviruses to transfer into epidemic amplification cycles. 

In cooperation with Prof. Ronald van Rij (Radboud University Nijmegen Medical Centre, the Netherlands)

Funded by DFG Priority Program ‘Ecology and species barriers in emerging viral diseases’ (SPP 1596, Junglen JU 2857/1-2)

 

Local diversity of host populations as determinants of reservoir-borne virus dynamics

Despite considerable research efforts during recent years, still very little is known about fundamental ecological mechanisms driving virus prevalence, evolutionary potential and emergence from wildlife reservoirs in general. Holistic approaches integrating host and viral traits are required to understand the dynamics and drivers of virus infections. This collaborative, multi-taxa project investigates the impact of anthropogenic habitat disturbance on host community composition, abundance pattern and immune genetic constitution of generalist species and relates the obtained data to local virus prevalence. Our aim is to identify ecological, behavioral and genetic constraints associated with virus evolution under the hypothesis of the dilution effect. We are focusing on three major viral vertebrate host taxa - bats, rodents and marsupials -, as well as on putatively blood-feeding mosquito vectors. Whereas our focus in the first funding period was on taking baseline data and parameter correlations, we will shift in the second period to ecological validation, ecological modeling and network analyses of host-virus systems in order to investigate the development and dynamics of virus mutation hotspots in disturbed landscapes. The central task will be to understand the processes and mechanisms that determine how biodiversity loss and shifts in species abundance of resilient, less sensitive species modulate virus ecology and lead to an increased prevalence and diversification of emerging pathogens within native animal communities.

 In cooperation with Prof. Simone Sommer, Prof. Marco Tschapka (University of Ulm), Prof. Dr. Gerald Kerth (University of Greifswald)

Funded by DFG Priority Program ‘Ecology and species barriers in emerging viral diseases’ (SPP 1596, Junglen JU 2857/3-2)

 

Identification of virus transmission networks to control key arboviral diseases in Kenya

Vector-borne diseases account for an estimated 17% of infectious diseases and cause more than 1 million deaths annually. RNA viruses, transmitted by blood-feeding arthropods (arboviruses), pose a particularly high risk as they can easily spread to new geographic regions where they may cause serious epidemics in naïve populations, such as by Zika-, chikungunya-, dengue- and Rift Valley fever viruses. Despite this high relevance, little is known about enzootic maintenance cycles and prevalence even for medically important arboviruses in the African countries of origin. Moreover, research of transmission cycles has mainly been focused on the role of mosquitoes as arboviral vectors. Other blood-feeding arthropods, such as ticks, biting midges and sandflies, have received considerable less attention. Relevant agents like Schmallenberg virus, Crimean-Congo hemorrhagic fever virus and phleboviruses are transmitted by these vectors.

Situated at one of the most renowned African entomology institutes, this collaborative project aims at a comprehensive understanding of key arboviral diseases in Kenya, including the virus sources, the infection-related impacts on humans, as well as the societal conditions and consequences of arbovirus disease. A strong focus in all stages of research will be on training and capacity building for future independent work in an African research environment. The research will have a local and exemplary focus to enable efficient training and technology transfer, but will provide important outcomes due to the setting of the work in one of the key seeding regions of arbovirus diseases globally.

The key objectives are (i) to study the disease ecology of relevant arboviruses in blood-feeding vectors, livestock and peri-domestic wildlife, (ii) to determine the impact of arboviral disease on human health and local society, involving complementary approaches from the fields of laboratory-based epidemiology and social sciences, and (iii) to provide training, education, and well-targeted capacity building in disease ecology, veterinary medicine and agro-social sciences, as well as virology.

The generated data will promote the local control of key arboviral diseases and thereby limit the risk of spread of indigenous arboviruses to new geographic regions. 

In cooperation with Prof. Christian Borgemeister (Centre for Development Research, Bonn), Prof. Rosemary Sang (Kenya Medical Research Institute and International Centre of Insect Physiology and Ecology, Nairobi, Kenya), Prof. Baldwyn Torto, Dr. David Tchouassi (International Centre of Insect Physiology and Ecology, Nairobi, Kenya)

Funded by the DFG (Junglen JU 2857/9-1)

 

Future Infections: Linking social-ecological transformation and arthropod-borne infections

(Part of the Collaborative Research Center 228: Future Rural Africa: Future-making and social-ecological transformation; www.futureruralafrica.de and www.crc228.de). 

Conservation and intensification are two important pathways of large-scale land-use change in rural Africa. Besides anticipated effects, these top-down strategies of future-making may have unintended side effects, in particular because of so called emerging infectious diseases (EIDs). These pose a serious threat to global health, and among the latter a significant number are arthropod-borne viruses, so-called arboviruses. Arboviruses are particularly sensitive to ecological changes and can easily escape from their natural transmission cycles and adapt to new hosts and geographic regions. Most prominent examples are the epidemics caused by yellow fever, Dengue, Chikungunya and Zika viruses in humans and Rift Valley fever (RVF) in both humans and animals. Land-use changes can have profound effects on biodiversity and EIDs. However, functional linkages between biodiversity and viral pathogens are poorly understood. Moreover, land-use, climatic and demographic changes and rapidly developing livestock markets with concurrent social transformations have a tremendous impact on the livestock sector, in particular on traditional forms of pastoralism in Africa. Yet the impact of ongoing socio-ecological transformations in rural Africa on arbovirus transmission cycles remains elusive. This project aims to understand how ecosystem diversity influences vector and virus populations in the light of large-scale land use changes in rural Africa. Effects of conservation and agricultural intensification (with a particular focus on invasive plant species) on selected arboviruses will be studied in the KAZA and the KRV sites. We will analyze major ecosystem characteristics and assess vector diversity and concomitant arbovirus infections at sites with different duration of land-use changes, the latter especially in KAZA. To identify if simple proxies of diversity can predict patterns of arbovirus abundance or prevalence, we will quantify the taxonomic diversity of vectors, hosts and vegetation. The latter could be important, as there are indications that some vector species favor certain invasive plants. Thus, this project will attempt to quantify the proportion of selected invasive plants, initially in the KRV site, and analyze the impact on vector dynamics and arbovirus prevalence. Close collaboration with projects studying agricultural futures (A03) and conservation areas (A04), ecosystem diversity and services (A01), the challenges of invasive species (B01), and the economic and energy transformations (C02) to name a few will enable a broad and long-term understanding on how such socio-ecological changes will affect arboviral diseases at the KAZA and KRV sites. 

In collaboration with Prof. Dr. Christian Borgemeister, Center for Development Research (ZEF), University of Bonn, Bonn, Germany; Dr. David Tchouassi, International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya; Dr. Rosemary Sang, Kenya Medical Research Institute, Nairobi, Kenya; Dr. John Mfune, Head of the Department of Biological Sciences, University of Namibia (UNAM), Namibia

Funded by the DFG Collaborative Research Center 228. 

 

Ecology of emerging arboviruses

Vector-borne infections account for about one fifth of all infectious diseases. Especially mosquito-borne viruses that often originate in tropical areas manage to spread to new hosts or new geographic regions with relative ease. However, our knowledge of arbovirus diversity, evolution, and geographic spread is mainly based on epidemic variants. To fill critical gaps in our knowledge on pre-epidemic viruses and viral emergence, we will characterize arbovirus diversity along tropical disturbance gradients to document and dissect microevolutionary processes in viral habitat expansion.

The overall aims of the group are: (i) to provide a comprehensive sample of mosquitoes from ecologically undisturbed and adjacent disturbed habitats in three main tropical rainforest ecosystem types occurring in Africa. We will characterise the true taxonomic diversity of arthropod-associated viruses including novel prototypic deep lineages still existing in natural ecosystems using state of the art methods; (ii) to identify the interplay between host community composition and viral emergence processes using mosquito-associated viruses as a multi-host and multi-pathogen system; and (iii) to analyse whether and how arbovirus emergence is driven by positive selection involving fitness gains of emergent strains using genetic and phenotypic viral population studies. Taken together we will obtain the first comprehensive assessment of viral microevolution during emergence in a real-world scenario.

Funded by the Federal Ministry of Education and Research (BMBF, Junglen 01KI1716)

 

 

Collaborators

  • Dr. Benon Assiimwe, Makerere University, Kampala, Uganda
  • Dr. Dennis A. Bente, University of Texas Medical Branch, Galveston, Texas, USA
  • Prof. Christian Borgemeister, Centre for Development Research (ZEF), Bonn, Germany
  • Dr. Thomas Gillespie, Emory University, Atlanta, USA
  • Prof. Bernhard Misof, Centre for Molecular Biodiversity Research (ZMB), Bonn, Germany
  • PD Dr. Lars Podsiadlowski, Institut für Evolutionsbiologie und Zooökologie, Universität Bonn, Bonn, Germany
  • Dr. Innocent Rwego, Makerere University, Kampala, Uganda
  • Prof. Rosemary Sang, Kenya Medical Research Insitute (KEMRI), Nairobi, Kenya
  • Dr. David Tchouassi, International Centre of Insect Phsyiology and Ecology (icipe), Nairobi, Kenya
  • Prof. Baldwyn Torto, International Centre of Insect Phsyiology and Ecology (icipe), Nairobi, Kenya
  • Prof. Dr. Ronald Van Rij, Radboud University Nijmegen Medical Centre, the Netherlands
  • Dr. Eric Begeron, Centers for Disease Control and Prevention, Atlanta, GA, United States
  • Prof. John Mfune, University of Namibia, Windhoek, Namibia
  • Prof. Simone Sommer, University of Ulm
  • Prof. Marco Tschapka, University of Ulm

Significant publications

A current list of all publications can be found in the pubmed-List or on

Google Scholar.