AG Müller

Sie befinden sich hier:

AG Müller

Our research is dedicated to emerging zoonotic viruses (mainly corona- and lyssaviruses) and their interactions with variable hosts. We use cell culture models to investigate mechanisms that determine the capability of viruses to switch hosts with a focus on the species-specific intracellular defence mechanisms (e.g. the interferon response).

Staff

PhD students

  • MSc Jan Papies
  • MSc Andrea Sieberg
  • MSc Simon Schroeder

Master students

  • BSc Vincent Blaschke

Technicians

 

Alumni

  • Dr. Daniela Niemeyer
  • Dr. Benjamin Meyer
  • Dr. Susanne Biesold
  • PhD Tasnim Suliman
  • Dipl.-Biol. Daniel Ritz
  • MD BSc Robert Wollny
  • MSc Hanna Roth
  • MSc Schwiedhard Hoffstall
  • MSc Eva-Maria Klein

Projects

We want to elucidate differences in the innate immune system of variable vertebrate hosts. In particular, we are interested in the influence of the host-specific defence mechanisms on the capability of viruses to switch hosts.

We use cell culture models from multiple vertebrate species and reverse genetic systems for coronaviruses (MERS and SARS-Coronavirus) to characterize and compare functions of distinct viral proteins in different host settings.

Our long-term goal is to identify crucial virus- and host-related factors that influence virulence, pathogenicity and transmissibility of viruses. By establishing a reporter cell-based screening platform we want to enable a high-throughput in-vitro risk assessment of yet uncharacterized viruses.

Methods we perform in the lab: Generation of primary and immortalized cell cultures, generation of reporter cell lines by CRISPR/Cas9, luciferase promoter assays, variety of transcriptome/ proteome analyses (e.g. real-time PCR, Interferon bioassays, ELISA, Immunofluorescence assays, Immunoprecipitations, FACS, SDS-PAGE and Western blotting).

External collaborators

  • Prof. Andreas Pichlmair, MPI for Biochemistry, Munich
  • Prof. Friedemann Weber, University of Gießen
  • Prof. Georg Kochs, University of Freiburg
  • Prof. Alexander Pfeifer, University of Bonn
  • Prof. Volker Thiel, Dr. Ronald Dijkman, Bern, Switzerland
  • Prof. Stefan Pöhlmann, Dr. Markus Hoffmann, DPZ, Göttingen
  • Prof. Susan Baker, Loyola University, Chicago, USA

Significant publications