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Our research is dedicated to emerging zoonotic viruses (mainly corona- and lyssaviruses) and their interactions with variable hosts. We use cell culture models to investigate mechanisms that determine the capability of viruses to switch hosts with a focus on the species-specific intracellular defence mechanisms (e.g. the interferon response).
- MSc Jan Papies
- MSc Andrea Sieberg
- MSc Simon Schroeder
- Dr. Daniela Niemeyer
- Dr. Benjamin Meyer
- Dr. Susanne Biesold
- PhD Tasnim Suliman
- Dipl.-Biol. Daniel Ritz
- MD BSc Robert Wollny
- MSc Hanna Roth
- MSc Schwiedhard Hoffstall
- MSc Eva-Maria Klein
We want to elucidate differences in the innate immune system of variable vertebrate hosts. In particular, we are interested in the influence of the host-specific defence mechanisms on the capability of viruses to switch hosts.
We use cell culture models from multiple vertebrate species and reverse genetic systems for coronaviruses (MERS and SARS-Coronavirus) to characterize and compare functions of distinct viral proteins in different host settings.
Our long-term goal is to identify crucial virus- and host-related factors that influence virulence, pathogenicity and transmissibility of viruses. By establishing a reporter cell-based screening platform we want to enable a high-throughput in-vitro risk assessment of yet uncharacterized viruses.
Methods we perform in the lab: Generation of primary and immortalized cell cultures, generation of reporter cell lines by CRISPR/Cas9, luciferase promoter assays, variety of transcriptome/ proteome analyses (e.g. real-time PCR, Interferon bioassays, ELISA, Immunofluorescence assays, Immunoprecipitations, FACS, SDS-PAGE and Western blotting).
- Prof. Andreas Pichlmair, MPI for Biochemistry, Munich
- Prof. Friedemann Weber, University of Gießen
- Prof. Georg Kochs, University of Freiburg
- Prof. Alexander Pfeifer, University of Bonn
- Prof. Volker Thiel, Dr. Ronald Dijkman, Bern, Switzerland
- Prof. Stefan Pöhlmann, Dr. Markus Hoffmann, DPZ, Göttingen
- Prof. Susan Baker, Loyola University, Chicago, USA